UL-424

Material Data Sheet

His6-GATE-16/Apg8p2 Fluorescein

Price: $175.00
Catalog #: UL-424

His6-GATE-16/Apg8p2 Fluorescein

Price: 
$175
Quantity: 
50 μg
Data Sheet: 
application/pdf iconUL-424_fluor GATE16.pdf
Stock: 

X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.

MW: 
15.7 kDa
Purity: 
> 95% by PAGE

Apg8p2 modified with fluorescein via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated Apg8p2 can be detected using avidin-linked reagents for higher efficiency and detection sensitivity than with antibodies. This protein is Nterminally His6-tagged.

Use & Storage

Use: 
Fluorescein- His6-Apg8p2 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively. 
Storage: 
Store at -80°C. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Becher P., et al. (2002) J. Virol. 76: 13069-13076
Elazar Z., et al. (2001) Genomics 74: 408-413
Paz Y., et al. (2000) J. Biol. Chem. 275: 25445-2545-
Sagiv Y., et al. (2000) EMBO J. 19: 1494-1504
Scherz-Shouval R., et al. (2003) J. Biol. Chem. 278: 14053-14058
Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744
Xin Y., et al. (2001) Genomics 74: 408-413