UL-420

Material Data Sheet

His6-GATE16

Price: $159.00
Catalog #: UL-420

His6-GATE16

Price: 
$159
Quantity: 
500 µg

Also known as: GABARAPL2

There are at least three groups of mammalian Apg8 proteins which are homologs of the yeast Atg8 protein, GATE-16 (Golgi-associated ATPase Enhancer of 16 kDa). The mammalian Apg8 proteins are ubiquitin-like modifiers that have divergent functions in humans, and are essential in autophagic conjugation systems. This modifier protein has a conserved C-terminal glycine residue that becomes covalently attached to phosphatidylethanolamine (PE) after it is activated by the Apg7p (E1) and Apg3p (E2) enzymes. GATE-16 is highly conserved from yeast to mammals, is constitutively expressed in secretory organs and localizes to the Golgi apparatus. The protein participates in membrane-trafficking events, and it has been proposed that GATE-16
modulates intra-Golgi transport by acting as an adaptor protein that couples NSF activity and SNARE activation. Accession # NP_009216.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.
MW: 
15.7 kDa
Purity: 
> 95% by PAGE

Use & Storage

Use: 

Typical concentration to support conjugation in vitro is 10-50 μM depending on
conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Becher P., et al. (2002) J. Virol. 76: 13069-13076
Elazar Z., et al. (2001) Genomics 74: 408-413
Paz Y., et al. (2000) J. Biol. Chem. 275: 25445-2545-
Sagiv Y., et al. (2000) EMBO J. 19: 1494-1504
Scherz-Shouval R., et al. (2003) J. Biol. Chem. 278: 14053-14058
Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744
Xin Y., et al. (2001) Genomics 74: 408-413