Material Data Sheet
Also known as: GABARAPL2
There are at least three groups of mammalian Apg8 proteins which are homologs of the yeast Atg8 protein, GATE-16 (Golgi-associated ATPase Enhancer of 16 kDa). The mammalian Apg8 proteins are ubiquitin-like modifiers that have divergent functions in humans, and are essential in autophagic conjugation systems. This modifier protein has a conserved C-terminal glycine residue that becomes covalently attached to phosphatidylethanolamine (PE) after it is activated by the Apg7p (E1) and Apg3p (E2) enzymes. GATE-16 is highly conserved from yeast to mammals, is constitutively expressed in secretory organs and localizes to the Golgi apparatus. The protein participates in membrane-trafficking events, and it has been proposed that GATE-16
modulates intra-Golgi transport by acting as an adaptor protein that couples NSF activity and SNARE activation. Accession # NP_009216.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.
Purity:> 95% by PAGE
Use & Storage
Typical concentration to support conjugation in vitro is 10-50 μM depending on
Store at -80°C. Avoid multiple freeze/ thaw cycles.
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