UL-416

Material Data Sheet

His6-GABARAP/Apg8p1 Rhodamine

Price: $175.00
Catalog #: UL-416

His6-GABARAP/Apg8p1 Rhodamine

Price: 
175
Quantity: 
50 µg
Data Sheet: 
application/pdf iconul-416-his6-gabarapapg8p1-rhodamine.pdf

Apg8p1 modified with rhodamine red via primary amine coupling, resulting in the modification of lysine residues as well as the N-terminus. This labeled Apg8p1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
16 kDa
Purity: 
> 95% by PAGE

Use & Storage

Use: 

Rhodamine Apg8p1 gives a strong signal in the range of 0.1-1 μM, depending
on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored
using Ex570 nm and Em590 nm wavelengths respectively. 

Storage: 
Store at -80°C. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Bavro V.N., et al. (2002) EMBO. Rep. 3: 183-189
Kouno T., et al. (2002) J. Biomol. NMR. 22: 97-98
Knight D., et al. (2002) J.Biol.Chem. 277: 5556-5561
Nymann-Anderson J., et al. (2002) Neuropharm. 43: 476-481
Tanida I., et al. (2003) Biochem. Biophys. Res. Comm. 300: 637-644
Stangler T., et al. (2002) J.Biol.Chem. 277: 15563-13366
Wang H., et al. (1999) Nature. 397: 69-72
Xin Y., et al. (2001) Genomics. 74: 408-413