UL-415

Material Data Sheet

GABARAP/Apg8p1 Agarose

Price: $150.00
Catalog #: UL-415

GABARAP/Apg8p1 Agarose

Price: 
$150
Quantity: 
0.5 ml
Data Sheet: 
application/pdf iconUL-415.pdf
Stock: 

0.5 ml of GABARAP/Apg8p1 agarose is supplied in a 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

GABARAP covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of GABARAP interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein. These include the Apg8 E1 activating enzyme Apg7, the Apg8 conjugating enzyme Apg3, Apg8 processing enzyme Apg4 and other autophagy pathway proteins and enzymes.

Use & Storage

Use: 

Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage: 
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

Literature

References: 

Bavro V.N. et al. (2002) EMBO. Rep. 3: 183-189
Klionsky D.J. (2005) J. Cell. Sci. 118: 7-18
Kouno T. et al. (2002) J. Biomol. NMR. 22: 97-98
Knight D. et al. (2002) J.Biol.Chem. 277: 5556-5561
Nymann-Anderson J. et al. (2002) Neuropharm. 43: 476-481
Tanida I. et al. (2003) Biochem. Biophys. Res. Comm. 300: 637-644
Stangler T. et al. (2002) J.Biol.Chem. 277: 15563-13366
Wang H. et al. (1999) Nature. 397: 69-72
Xin Y. et al. (2001) Genomics. 74: 408-413