Material Data Sheet


Price: $159.00
Catalog #: UL-415


500 µL
Data Sheet: 

GABARAP covalently coupled to agarose beads via primary amines allowing for a fully
functional C-terminus. Useful for isolation and capture of GABARAP interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein. These include the Apg8 E1 activating enzyme Apg7, the Apg8 conjugating enzyme Apg3, Apg8 processing enzyme Apg4 and other autophagy pathway proteins and enzymes.

Product Information

 0.5 ml of GABARAP/Apg8p1 agarose is supplied in a 1 ml total volume of 50 mM Hepes pH 7.5, 250 mM NaCl.

Use & Storage


Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.


The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.



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