UL-414

Material Data Sheet

His6-GABARAP Fluorescein

Price: $195.00
Catalog #: UL-414

His6-GABARAP Fluorescein

Price: 
$195
Quantity: 
50 µg

Apg8p1 modified with fluorescein via primary amine coupling resulting in the modification of lysine residues as well as the N-terminus. This labeled Apg8p1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.

Product Information

Stock: 
X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.
MW: 
16 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Fluorescein- His6-Apg8p1 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively. 

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bavro V.N., et al. (2002) EMBO. Rep. 3: 183-189
Kouno T., et al. (2002) J. Biomol. NMR. 22: 97-98
Knight D., et al. (2002) J.Biol.Chem. 277: 5556-5561
Nymann-Anderson J., et al. (2002) Neuropharm. 43: 476-481
Tanida I., et al. (2003) Biochem. Biophys. Res. Comm. 300: 637-644
Stangler T., et al. (2002) J.Biol.Chem. 277: 15563-13366
Wang H., et al. (1999) Nature. 397: 69-72
Xin Y., et al. (2001) Genomics. 74: 408-413