UL-410

Material Data Sheet

His6-GABARAP

Price: $159.00
Catalog #: UL-410

His6-GABARAP

Price: 
$159
Quantity: 
500 µg

There are at least three groups of mammalian Apg8 proteins which are homologs of the yeast Atg8 protein, including GABARAP (GABAA Receptor Associated Protein). The mammalian Apg8 proteins are ubiquitin-like modifiers that have divergent functions in human, and are essential in autophagic conjugation systems. This modifier protein has a conserved C-terminal glycine residue that becomes covalently attached to phosphatidylethanolamine (PE) after it is activated by the Apg7p (E1) and Apg3p (E2) enzymes. GABARAP is associated with intracellular membranes and co-localizes with microtubules. GABARAP may play a role in intracellular transport and clustering of neurotransmitters (like GABAA receptors) by mediating their interaction with the cytoskeleton. Accession # NP_009209.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific Lot #.
MW: 
16 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bavro V.N., et al. (2002) EMBO. Rep. 3: 183-189
Kouno T., et al. (2002) J. Biomol. NMR. 22: 97-98
Knight D., et al. (2002) J.Biol.Chem. 277: 5556-5561
Nymann-Anderson J., et al. (2002) Neuropharm. 43: 476-481
Tanida I., et al. (2003) Biochem. Biophys. Res. Comm. 300: 637-644
Stangler T., et al. (2002) J.Biol.Chem. 277: 15563-13366
Wang H., et al. (1999) Nature. 397: 69-72
Xin Y., et al. (2001) Genomics. 74: 408-413