Material Data Sheet

Di-Ub (K11-linked) FRET TAMRA Pos4

Price: $0.00
Catalog #: UF-440

Di-Ub (K11-linked) FRET TAMRA Pos4

50 µg

Linkage specific diubiquitin is a substrate for enzymes that cleave the isopeptide linkage between two ubiquitin molecules. These FRET-based DUB substrates with isopeptide bonds between ubiquitin moieties are superior to gel densitometry assays. Since DUBs recognize and cleave substrates with specific steric conditions, each substrate varies in the position of the fluorophore and quencher and must be empirically tested for individual enzymes. Ubiquitination through K11is important for endoplasmic reticulum-associated degradation (ERAD) and there undoubtedly exist DUBs that may have specificity for K11 linkages.

Product Information

 X mg/ml (X μM) in 50 mM Hepes pH 7.5, 150 mM NaCl, 2 mM DTT. Concentration varies with lot number.
17 kDa
> 95% by SDS-PAGE

Use & Storage


Typical substrate concentrations range from 100-500 nM and typical enzymeconcentrations range from 0.5-10 nM but depend on specific assay conditions and method of detection. Recommended assay buffer is 50 mM Hepes pH 7.5,150 mM NaCl, 2 mM DTT. Fluorescence can be monitored using Ex544 nm and Em572 nm wavelengths.


Store at -80°C. Avoid multiple freeze/thaw cycles.



Bish R.A. et al. (2008) J. Proteome Res. 7:3481-3489

Ikeda F. and Dikic I. (2008) EMBO Rep. 9:536-542

Fushman D. and Walker O. (2009) J. Mol. Biol.

Jin L. et al. (2008) Cell. 133:653-665

Li W. and Ye Y. (2008) Cell. Mol. Life.Sci. 65:2397-2496

Lingyan J. et al. (2009) Cell. 133:653-665

Pickart C.M. and Fushman D. (2004) Curr.Opin. Chem. Biol. 8:610-616

Xu P., et al. (2009) Cell. 137:133-145