UF-230

Material Data Sheet

Di-Ub (K48-linked) FRET TAMRA Pos3

Price: $250.00

Catalog #: UF-230

Di-Ub (K48-linked) FRET TAMRA Pos3

Price:

250

Quantity:

50 µg

Data Sheet:

uf-230-di-ub-k48-linked-fret-tamra-pos3.pdf

Linkage specific diubiquitin is a substrate for enzymes that cleave the isopeptide linkage between two ubiquitin molecules. These FRET-based DUB substrates with isopeptide bonds between ubiquitin moieties are superior to gel densitometry assays. Since DUBs recognize and cleave substrates with specific steric conditions, each substrate varies in the position of the fluorophore and quencher and must be empirically tested for individual enzymes.

Product Information

Stock:

X mg/ml (X μM) in 50 mM Hepes pH 7.5, 150 mM NaCl, 2 mM DTT. Concentration varies with lot number.

MW:

17 kDa

Purity:

> 95% by SDS-PAGE

Use & Storage

Use:

Typical substrate concentrations range from 100-500 nM and typical enzymeconcentrations range from 0.5-10 nM but depend on specific assay conditionsand method of detection. Recommended assay buffer is 50 mM Hepes pH 7.5,150 mM NaCl, 2 mM DTT. Fluorescence can be monitored using Ex544nmand Em572 nm wavelengths.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.