UCN-712

Material Data Sheet

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (Linear), Agarose

Price: $215.00
Catalog #: UCN-712

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (Linear), Agarose

Price: 
$215
Quantity: 
250 ┬ÁL

This linear ubiquitin fusion protein is resistant to the activity of enzymes (DUB's) that cleave the peptide linkage between adjacent ubiquitin molecules.  Ub is not expressed directly as free Ub, but rather as linear fusions either to itself or to certain ribosomal protein subunits.  These Ub-fusion precursors are proteolyzed by deubiquitinating enzymes (DUB's) at the appropriate junction points to yield active Ub monomers with C-termini ending in GG.  There are likely several intracellular DUB's which perform this essential processing role.  This product may be useful in analyzing interactions between linear ubiquitin and proteins that contain ubiquitin-associated domains (UBA's) or ubiquitin-interacting motifs (UIM's).

Product Information

Stock: 
Non-hydrolyzable Ub4 (linear) chains coupled to beads at 2 mg/ml (58 nmol/ml).

Use & Storage

Use: 

Prepare resin by rinsing with 10 volumes of water to remove ethanol storage buffer.  Equilibriate resin by washing with 10 volumes of desired start buffer.  Binding and elution of material is dependent on individual experimental conditions and requirements.

Storage: 
Polyubiquitin-agarose can be re-used if properly maintained. After use, clean resin with a wash cycle of 5 volumes 100 mM HEPES pH 8.0, 500 mM NaC1 followed by 5 volumes 100 mM NaOAc pH 4.5, 500 mM NaC1. Repeat twice, then rinse resin with a low salt buffer. Store resin at 4 degrees celsius in neutral aqueous buffer containing 1 mM NaN3 or 20% ethanol as a preservative. DO NOT FREEZE.

Literature

References: 

Gerlach B., et al (2011) Nature 471: 591-596

Emmerich C., et al (2011) Science Signaling 4: re5

Sato Y., et al (2011) PNAS 108: 20520-20525