UCN-710

Material Data Sheet

Tetra-Ub/Ub4, Non-Hydrolyzable Chains, (Linear)

Price: $129.00
Catalog #: UCN-710

Tetra-Ub/Ub4, Non-Hydrolyzable Chains, (Linear)

Price: 
$129
Quantity: 
100 µg

This linear ubiquitin fusion protein is resistant to the activity of enzymes (DUB's) that cleave the peptide linkage between adjacent ubiquitin molecules.  Ub is not expressed directly as free Ub, but rather as linear fusions either to itself or to certain ribosomal protein subunits.  These Ub-fusion precursors are proteolyzed by deubiquitinating enzymes (DUB's) at the appropriate junction points to yield active Ub monomers with C-termini ending in GG.  There are likely several intracellular DUB's which perform this essential processing role.  This protein may be useful in analyzing interactions between linear ubiquitin and proteins that contain ubiquitin-associated domains (UBA's) or ubiquitin-interacting motifs (UIM's).

Product Information

MW: 
34 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 
-20 degrees celsius. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gerlach B., et al. (2011) Nature 471: 591-596

Emmerich C., et al. (2011) Science Signaling 4: re5

Sato Y., et al. (2011) PNAS 108: 20520-20525