UCN-40

Material Data Sheet

Di-Ub/Ub2 Non-Hydrolyzable Chains (K11-linked)

Price: $89.00
Catalog #: UCN-40

Di-Ub/Ub2 Non-Hydrolyzable Chains (K11-linked)

Price: 
$89
Quantity: 
25 μg

Linkage specific, non-hydrolyzable di-ubiquitin is resistant to the activity of enzymes (DUB’s) that cleave the isopeptide linkage between adjacent ubiquitin molecules. It can be used to investigate binding interactions between di-ubiquitin and proteins that contain elements such as ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Biological roles of K11-linked polyubiquitin are less understood than K48- or K63-linked Ub chains, though recent evidence points to a role for K11 linkages in the degradation of the anaphase-promoting complex (APC/C), and in TNFa-stimulated NF-kB activation.

Product Information

MW: 
17 kDa
Purity: 
> 95 % by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Aqueous solution at –20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Wickliffe K.E., et al. (2011) Trends Cell Biol.   21: 656-63

Dynek J.N., et al. (2010) EMBO J.  29: 4198-4209

Komander D.  (2009)  Biochem Soc Trans  37: 937–953

Jin L., et. al. (2008)  Cell  133: 653-665