Material Data Sheet

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (K63-linked), Agarose

Price: $195.00
Catalog #: UCN-312

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (K63-linked), Agarose

100 ┬ÁL

Linkage specific, non-hydrolyzable tetra-ubiquitin is resistant to the activity of enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules.  It can be used to investigate binding interactions between tetra-ubiquitin and proteins that contain elements such as ubiquitin-associated domains (UBA's), ubiquitin-interacting motifs (UIM's), and CARD domains.  This product may also be useful in exploring the role of unanchored poly-ubiquitin chains in some signaling pathways.

Product Information

Non-hydrolyzable Ub4 (K63) chains coupled to beads at 1 mg/ml (29 nmol/ml).

Use & Storage


Prepare resin by rinsing with 10 volumes of water to remove ethanol storage buffer.  Equilibriate resin by washing with 10 volumes of desired start buffer.  Binding and elution of material is dependent on individual experimental conditions and requirements.

Polyubiquitin-agarose can be re-used if properly maintained. After use, clean resin with a wash cycle of 5 volumes 100 mM HEPES pH 8.0, 500 mM NaC1 followed by 5 volumes 100 mM NaOAc pH 4.5, 500 mM NaC1. Repeat twice, then rinse resin with a low salt buffer. Store resin at 4 degrees celsius in neutral aqueous buffer containing 1 mM NaN3 or 20% ethanol as preservative. DO NOT FREEZE.



Brasher B., et al. (2012) Poster presented at the Sixth International Conference - SUMO, Ubiquitin, UBL Proteins: Implications for Human Diseases, Houston, TX.

Zeng W., et al. (2010) Cell 141: 315-330

Kulathu Y., et al. (2009) Nat. Struct Mol Biol. 16: 1328-1330

Komander D. (2009) Biochem Soc Trans 37: 937-953