UCN-310

Material Data Sheet

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (K63-linked)

Price: $149.00
Catalog #: UCN-310

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (K63-linked)

Price: 
$149
Quantity: 
25 µg

Linkage specific, non-hydrolyzable tetra-ubiquitin is resistant to the activity of enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules.  It can be used to investigate binding interactions between tetra-ubiquitin and proteins that contain elements such as ubiquitin-associated domains (UBA's), ubiquitin-interacting motifs (UIM's) and CARD domains.  This product may also be useful in exploring the role of unanchored poly-ubiquitin chains in some signaling pathways.

Product Information

MW: 
34 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 
Aqueous solution at -20 degrees celsius. Avoid multiple freeze/thaw cycles.

Literature

References: 

Brasher B. et al. (2012) Poster session presented at the Sixth International Conference - SUMO, Ubiquitin, UBL Proteins: Implications for Human Diseases, Houston, TX.

Kulathu Y., et al. (2009) Nat. Struct Mol biol. 16: 1328-1330

Zeng W., et al. (2010) Cell 141: 315-330