UCN-302

Material Data Sheet

Di-Ub/Ub2 Non-Hydrolyzable Chains (K63-linked), Agarose

Price: $215.00
Catalog #: UCN-302

Di-Ub/Ub2 Non-Hydrolyzable Chains (K63-linked), Agarose

Price: 
$215
Quantity: 
250 μL

Linkage specific, non-hydrolyzable di-ubiquitin is resistant to the activity of enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules. It can be used to investigate binding interactions between di-ubiquitin and proteins that contain elements such as ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). This product may also be useful in exploring the role of unanchored poly-ubiquitin chains in some signaling pathways. 

Use & Storage

Use: 

Prepare resin by rinsing with 10 volumes of water to remove ethanol storage buffer. Equilibrate resin by washing with 10 volumes of desired start buffer. Binding and elution of material is dependent on individual experimental conditions and requirements. 

Storage: 
Polyubiquitin-agarose can be re-used if properly maintained. After use, clean resin with a wash cycle of 5 volumes 100 mM HEPES pH 8.0, 500 mM NaCl followed by 5 volumes 100 mM NaOAc pH 4.5, 500 mM NaCl. Repeat twice, then rinse resin with a low salt buffer. Store resin at 4°C in neutral aqueous buffer containing 1 mM NaN3 or 20% ethanol as preservative. DO NOT FREEZE.

Literature

References: 

Brasher B., et al. (2012) Poster presented at the Sixth International Conference-SUMO, Ubiquitin, UBL Protwins: Implications for Human Diseases, Houston, TX.

Zeng W., et al. (2010) Cell 141: 315-330

Komander D. (2009) Biochem Soc Trans 37: 937-953

Kulathu Y., et al. (2009) Nat. Struct Mol Biol. 16: 1328-1330