UCN-300

Material Data Sheet

Di-Ub/Ub2 Non-Hydrolyzable Chains (K63-linked)

Price: $139.00
Catalog #: UCN-300

Di-Ub/Ub2 Non-Hydrolyzable Chains (K63-linked)

Price: 
$139
Quantity: 
100 μg

Linkage specific, non-hydrolyzable di-ubiquitin is resistant to the activity of enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules. It can be used to investigate binding interactions between di-ubiquitin and proteins that contain elements such as ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). This product may also be useful in exploring the role of unanchored poly-ubiquitin chains in some signaling pathways. 

Product Information

MW: 
17 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 
Aqueous solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Brasher B., et al. (2012) Poster presented at the Sixth International Conference-SUMO, Ubiquitin, UBL Proteins: Implications for Human Diseases, Houston, TC.

Zeng W., et al. (2010) Cell 141: 315-330

Kulathu Y.,  et al. (2009) Nat. Struct Mol Biol. 16: 1328-1330