UCN-215

Material Data Sheet

Tri-Ub/Ub3 Non-Hydrolyzable Chains (K48-linked)

Price: $105.00
Catalog #: UCN-215

Tri-Ub/Ub3 Non-Hydrolyzable Chains (K48-linked)

Price: 
$105
Quantity: 
25 μg

Linkage specific, non-hydrolyzable tri-ubiquitin is resistant to the activity of enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules. It can be used to investigate binding interactions between tri-ubiquitin and proteins that contain elements such as ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). This product may also be useful in exploring the role of unanchored poly-ubiquitin chains in some signaling pathways. 

Product Information

MW: 
26 kDa
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentration will depend on specific assay conditions and method of detection.

Storage: 
Aqueous solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Brasher B., et al. (2012) Poster presented at the Sixth International Conference-SUMO, Ubiquitin, UBL Proteins: Implications for Human Diseases, Houston, TX.

Zhang D., et al. (2008) J. Mol. Biology 377: 162-180