UCN-212

Material Data Sheet

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (K48-linked), Agarose

Price: $195.00
Catalog #: UCN-212

Tetra-Ub/Ub4 Non-Hydrolyzable Chains (K48-linked), Agarose

Price: 
$195
Quantity: 
100 μL

Linkage specific, non-hydrolyzable tetra-ubiquitin is resistant to the activity of enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules. It can be used to investigate binding interactions between ubiquitin chains and proteins that contain elements such as ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Tetra-ubiquitin is the minimal unit necesary for recognition by the 26S proteasome and contains structural characteristics (such as repeating hydrophobic patches) not present in di-ubiquitin. This product may also be useful in exploring the role of unanchored poly-ubiquitin chains in some signaling pathways. 

Product Information

Stock: 
Non-hydrolyzable Ub4 (K48) chains coupled to beads at 1 mg/ml (29 nmol/ml).

Use & Storage

Use: 

Prepare resin by rinsing with 10 volumes of water to remove ethanol storage buffer. Equilibrate resin by washing with 10 volumes of desired start buffer. Binding and elution of material is dependent on individual experimental conditions and requirements.

Storage: 
Polyubiquitin-agarose can be re-used if properly maintained. After use, clean resin with a wash cycle of 5 volumes 100 mM HEPES pH 8.0, 500 mM NaCl followed by 5 volumes 100 mM NaOAc pH 4.5, 500 mM NaCl. Repeat twice, then rinse resin with a low salt buffer. Store resin at 4°C in neutral aqueous buffer containing 1 mM NaN3 or 20% ethanol as preservative. DO NOT FREEZE.

Literature

References: 

Komander D. (2009) Biochem Soc Trans 37:937-953

Ventadour S, et al. (2007) J. Biol. Chem. 282: 5302-5309

Brasher B., et al. (2012) Poster presented at the Sixth International Conference-SUMO, Ubiquitin, UBL Proteins: Implications for Human Diseases, Houston, TX.

Tenno T., et al. (2004) Genes to Cells. 9:865-875