UCN-200

Material Data Sheet

DI-Ub/Ub2 Non-Hydrolyzable Chains (K48-linked)

Price: $139.00
Catalog #: UCN-200

DI-Ub/Ub2 Non-Hydrolyzable Chains (K48-linked)

Price: 
$139
Quantity: 
100 μg

Linkage specific, non-hydrolyzable di-ubiquitin is resistant to the activity of enzymes (DUB's) that cleave the isopeptide linkage between adjacent ubiquitin molecules. It can be used to investigate binding interactions between di-ubiquitin and proteins that contain elements such as ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). This product may also be useful in exploring the role of unanchored poly-ubiquitin chains in some signaling pathways.

Product Information

MW: 
17 kDa
Purity: 
>95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection. 

Storage: 
Aqueous solution at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Brasher B., et al.(2012) Poster session presented at the Sixth International Conference-Sumo, Ubiquitin, UBL Proteins: Implications for Human Diseases, Houston, TX.

Zhang D., et al. (2008) J.Mol.Biology 377: 167-180