UCM-310

Material Data Sheet

Tetra-Ubiquitin (Ub-K48-Ub-K63-Ub-K48-Ub) WT Chains

Price: $215.00
Catalog #: UCM-310

Tetra-Ubiquitin (Ub-K48-Ub-K63-Ub-K48-Ub) WT Chains

Price: 
$215
Quantity: 
25 µg
Tetra-Ub can be used to investigate mechanisms of binding and recognition by deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Tetra-Ub is the minimal unit necessary for recognition by the 26S proteasome and contains structural characteristic (such as repeating hydrophobic patches) not present in di-Ub. This product is made with wild-type human recombinant ubiquitin and linkage-specific enzymes, which results in one K63-linkage between two K48-linkages.

Product Information

MW: 
34 kDa
Purity: 
> 90% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Beal R.E., et al. (1995) Biochem. 37:2925-2934
Cook W. J., et al. (1992) J.Biol.Chem. 267:16467-16471
Cook W. J. et al. (1994) J.Mol.Biol. 236:601-609
Phillips C. L., et al. (2001) Acta.Cryst. 57:341-344
Piotrowski J. et al. (1997) J.Biol.Chem. 272:23712-23721
Tenno T., et al. (2004) Genes to Cells. 9:865-875
Wilkinson K.D., et al. (1995) Biochem. 34:14535-14546