UCH-230

Material Data Sheet

His6-Poly-Ubiquitin/Ub2-Ub7 WT Chains (K48-linked)

Price: $215.00
Catalog #: UCH-230

His6-Poly-Ubiquitin/Ub2-Ub7 WT Chains (K48-linked)

Price: 
$215
Quantity: 
100 µg

Linkage specific poly-ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys48- linked chains are abundant in vivo and act as a universal signal for proteasomal degradation. This product is formed with His6-tagged wild-type human recombinant ubiquitin and linkage-specific enzymes. This mixture of poly-ubiquitin chains contains di-ubiquitin and higher MW species; mono-ubiquitin has been removed. The His6-tag is convenient for metal chelate affinity purification and immuno-detection using His6-specific antibodies.

Product Information

Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Chan N., et al. (2001) Nature.Struc.Biol. 8:650-652
Piotrowski J., et al.. (1997) J.Biol.Chem. 272:23712-23721
Tenno T., et al.. (2004) Genes to Cells. 9:865-875
Throwe J.S., et al. (2000) EMBO.J. 19:94-102
Van Nocker S., et al.. (1993) J.Biol.Chem. 268:24766-24773
Wilkinson K.D., et al.. (1995) Biochem. 34:14535-14546