UCB-330

Material Data Sheet

Biotin Poly-Ubiquitin/Ub2-Ub7 WT Chains (K63-linked)

Price: $215.00
Catalog #: UCB-330

Biotin Poly-Ubiquitin/Ub2-Ub7 WT Chains (K63-linked)

Price: 
$215
Quantity: 
50 µg
Data Sheet: 

Poly-Ubiquitin chains are composed of Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule. Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).This mixture of poly-Ubiquitin chains contains di-Ubiquitin and higher MW species; mono-Ubiquitin has been removed. These chains have been modified with biotin via primary amine coupling, resulting in multiple biotinylated species modified at the N-terminus, as well as lysine residues. Biotinylated Ubiquitin can be detected using avidin-linked reagents. This mixture of poly-ubiquitin chains contains di-ubiquitin and higher MW species; mono-ubiquitin has been removed. These chains have been modified with biotin via primary amine coupling. This results in multiple biotinylated species modified at the N-terminus, as well as lysine residues. Biotinylated ubiquitin can be detected using avidin-linked reagents.

Product Information

Stock: 
X mg/ml in 50 mM HEPES pH 8.0
Purity: 
> 90% by SDS-PAGE
Storage: 

Store at -20°C to -70°C.  Avoid multiple freeze-thaw cycles