UCB-310

Material Data Sheet

Biotin Tetra-Ub/Ub4 WT Chains (K63-linked)

Price: $149.00
Catalog #: UCB-310

Biotin Tetra-Ub/Ub4 WT Chains (K63-linked)

Price: 
$149
Quantity: 
25 µg
Data Sheet: 

Linkage specific Tetra-Ub can also be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).  This product is formed with wild-type ubiquitin and linkage-specific enzymes. This product is made with wild-type human recombinant ubiquitin and linkage-specific enzymes. These chains are modified with biotin via primary amine coupling.  This results in multiple biotinylated species modified at the N-terminus, as well as lysine residues.  Biotinylated ubiquitin can be detected using avidin-linked reagents.

Product Information

Stock: 
lyophilized powder
MW: 
34 kDa
Purity: 
> 90% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Beal R.E., et al. (1995) Biochem.  37: 2925-2934

Cook W. J., et al. (1992) J. Biol. Chem.  267: 16467-16471

Cook W. J. et al. (1994) J. Mol. Biol. 236: 601-609

Phillips C. L., et al. (2001) Acta. Cryst. 57: 341-344

Piotrowski J. et al. (1997) J. Biol. Chem.  272: 23712-23721

Tenno T., et al. (2004) Genes to Cells.  9: 865-875

Wilkinson K.D., et al. (1995) Biochem.  34: 14535-14546