UCB-310
Biotin Tetra-Ub/Ub4 WT Chains (K63-linked)
Biotin Tetra-Ub/Ub4 WT Chains (K63-linked)
Linkage specific Tetra-Ub can also be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs). This product is formed with wild-type ubiquitin and linkage-specific enzymes. This product is made with wild-type human recombinant ubiquitin and linkage-specific enzymes. These chains are modified with biotin via primary amine coupling. This results in multiple biotinylated species modified at the N-terminus, as well as lysine residues. Biotinylated ubiquitin can be detected using avidin-linked reagents.
Product Information
Use & Storage
Typical concentrations will depend on specific assay conditions and method of detection.
Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.
Literature
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