UCB-230

Material Data Sheet

Biotin Poly-Ubiquitin/Ub2-Ub7 WT Chains (K48-linked)

Price: $215.00
Catalog #: UCB-230

Biotin Poly-Ubiquitin/Ub2-Ub7 WT Chains (K48-linked)

Price: 
$215
Quantity: 
50 µg

Linkage specific poly-ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).  Lys48-linked chains are abundant in vivo and act as a universal signal for proteasomal degradation.  This product is formed with wild-type human recombinant ubiquitin and linkage-specific enzymes.  This mixture of poly-ubiquitin chains contains di-ubiquitin and higher MW species; mono-ubiquitin has been removed. These chains have been modified with biotin via primary amine coupling.  This results in multiple biotinylated species modified at the N-terminus, as well as lysine residues.  Biotinylated ubiquitin can be detected using avidin-linked reagents.

Product Information

Stock: 
lyophilized powder
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.

Purity: 
> 90% by SDS-PAGE

Literature

References: 

 

Chan N., et al. (2001) Nature. Struc. Biol. 8: 650-652

Piotrowski J., et al. (1997) J. Biol. Chem. 272: 23712-23721

Tenno T., et al. (2004) Genes to Cells.  9: 865-875

Throwe J.S., et al. (2000) EMBO. J. 19: 94-102

Van Nocker S., et al. (1993) J. Biol. Chem. 268: 24766-24773

Wilkinson K.D., et al. (1995) Biochem. 34: 14535-14546