Material Data Sheet

Biotin Tetra-Ubiquitin/Ub4 WT Chains (K48-linked)

Price: $215.00
Catalog #: UCB-210

Biotin Tetra-Ubiquitin/Ub4 WT Chains (K48-linked)

25 µg


Linkage specific Tetra-Ub can also be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).  This product is formed with wild-type ubiquitin and linkage-specific enzymes.  Tetra-Ub is the minimal unit necessary for recognition by the 26S proteasome and contains structural characteristic (such as repeating hydrophobic patches) not present in di-Ub.  This product is made with wild-type human recombinant ubiquitin and linkage-specific enzymes. These chains are modified with biotin via primary amine coupling.  This results in multiple biotinylated species modified at the N-terminus, as well as lysine residues.  Biotinylated ubiquitin can be detected using avidin-linked reagents.

Product Information

lyophilized powder
34 kDa
> 90% by SDS-PAGE
Aqueous solutions up to 5 mg/ml

Use & Storage


Typical concentrations will depend on specific assay conditions and method of detection.


Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.




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