UC-330

Material Data Sheet

Poly-Ubiquitin/Ub2-Ub7 WT Chains (K63-linked)

Price: $219.00
Catalog #: UC-330

Poly-Ubiquitin/Ub2-Ub7 WT Chains (K63-linked)

Price: 
$219
Quantity: 
100 µg
Linkage specific poly-ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys63- linked poly-ubiquitin has been implicated in several non-degradative processes such as receptor endocytosis and sorting, translation, DNA damage repair, the stress response and signaling in the NFκB pathway. This product is formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. This mixture of poly-ubiquitin chains contains di-ubiquitin and higher MW species; mono-ubiquitin has been removed.

Product Information

Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Typical concentrations will depend on specific assay conditions and method of detection.

Storage: 

Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Chen Z.J., et al. (2004) Can.Biol.Ther 3:286-288
Deng L., et al. (2000) Cell 103:351-361
Hofman L., et al. (2001) J.Biol.Chem. 276:27936-43
Spence J., et al. (1995) Mol.Cell Biol. 15:1265-1273
Tenno T., et al. (2004) Genes to Cells. 9:865-875
Varadan R., et al. (2004) J Biol.Chem. 279:7055-7063