UC-320
Material Data Sheet
Poly-Ubiquitin/Ub3-Ub7 WT Chains (K63-linked)
Price: $219.00
Poly-Ubiquitin/Ub3-Ub7 WT Chains (K63-linked)
Price:
$219
Quantity:
100 µg Linkage specific poly-ubiquitin chains are used to investigate mechanisms of chain recognition,
binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins
that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys63-
linked poly-ubiquitin has been implicated in several non-degradative processes such as receptor
endocytosis and sorting, translation, DNA damage repair, the stress response and signaling in the
NFκB pathway. This product is formed with wild-type ubiquitin and linkage-specific enzymes.
This mixture of poly-ubiquitin chains contains tri-ubiquitin and higher MW species, mono- and
di-ubiquitin have been removed.
Product Information
Purity:
> 95% by SDS-PAGE
Solubility:
Aqueous solutions up to 5 mg/ml
Supplied:
This is a lyophilized powder Use & Storage
Use:
Typical concentrations will depend on specific assay conditions and method of detection.
Storage:
Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.
Literature
References:
Chan N., et al. (2001) Nature.Struc.Biol. 8:650-652
Piotrowski J., et al.. (1997) J.Biol.Chem. 272:23712-23721
Tenno T., et al.. (2004) Genes to Cells. 9:865-875
Throwe J.S., et al. (2000) EMBO.J. 19:94-102
Van Nocker S., et al.. (1993) J.Biol.Chem. 268:24766-24773
Wilkinson K.D., et al.. (1995) Biochem. 34:14535-14546