UC-316

Material Data Sheet

Penta-Ubiquitin/Ub5 WT Chains (K63-linked)

Price: $195.00
Catalog #: UC-316
Penta-Ubiquitin/Ub5 WT Chains (K63-linked)
Price: 
$195
Quantity: 
25 μg
Data Sheet: 
Linkage specific penta-Ub can be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases, the proteasome or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). This product is formed with wild-type ubiquitin and linkage-specific enzymes. It has been shown that the rate of ubiquitin-substrate conjugate degradation is related to poly-ubiquitin chain length. Tetra-ubiquitin is the minimal unit required for recognition by the proteasome, and longer chains probably have enhanced binding to proteasomal subunits and may be more resistant to disassembly by a proteasome-associated isopeptidases.

Product Information

MW: 
43 kDa
Purity: 
> 90% by SDS-PAGE
Solubility: 
Aqueous solutions up to 5 mg/ml

Use & Storage

Use: 
Typical concentrations will depend on specific assay conditions and method of detection.
Storage: 
Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles.
Purity: 
> 90% by SDS-PAGE

Literature

References: 

Beal R.E., et al. (1996) Proc. Natl. Acad. Sci. USA. 93:861-866

Beal R.E., et al. (1998) Biochem. 37:2925-2934

Deveraux Q., et al. (1994) J. Biol. Chem. 269:7059-7061

Piotrowski J., et al. (1997) J. Biol. Chem. 272:23712-21.

Wang Q., et al. (2005) J. Mol. Biol. 348:727-739