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Material Data Sheet
Tri-Ub/Ub3 WT Chains (K63-linked)
Price: $250.00
Tri-Ub/Ub3 WT Chains (K63-linked)
Price:
$250
Quantity:
100 μg
Data Sheet:
MW:
26 kDa
Purity:
> 95% by SDS-PAGE
Solubility:
Aqueous solutions up to 5 mg/ml
Supplied:
This is a lyophilized powder
Linkage specific tri-Ub can be used as a substrate for enzymes that cleave the isopeptide linkage
between ubiquitin molecules. It can also be used to investigate mechanism of binding and
recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that
contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). This
product is formed with wild-type human recombinant ubiquitin and linkage-specific enzymes.
Use & Storage
Use:
Typical concentrations will depend on specific assay conditions and method of detection.
Storage:
Solubilized solution at –20°C. Avoid multiple freeze/thaw cycles. Literature
References:
Buchberger A., et al. (2002) Trends.Cell.Biol. 12:216-221
Corsi D., et al. (1997) J.Biol.Chem. 272:2977-2983
Kim H. J., et al. (2003) J.Biochem. 134:9-19
Robinson P. A., et al. (2004) J.Cell.Sci 117:5191-5194
Takada K., et al. (1995) Eur.J.Biochem. 233:42-47
Takada K., et al. (2001) Biochem.J. 356:199-206






