Material Data Sheet

Tetra-Ub/Ub4 WT Chains (K48-linked), S65 phosphorylated

Price: $255.00
Catalog #: UC-250

Tetra-Ub/Ub4 WT Chains (K48-linked), S65 phosphorylated

25 µg
Data Sheet: 

Linkage specific phosphorylated poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Phosphorylated poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements.

K48-linked Tetra-Ubiquitin chains phosphorylated at Serine 65 are manufactured using recombinant wild-type human Ubiquitin, linkage-specific enzymes, and PINK1 kinase.  The use of purely enzymatic techniques avoids the potential for contaminating synthetic intermediates.  The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of poly-Ubiquitin samples.

Product Information

1 mg/ml (29 µM) in sterile, deionized water
34 kDa
>95% by SDS-PAGE, Ub-AQUA analysis provided for each lot

Store at –20°C. Avoid multiple freeze/thaw cycles.