Material Data Sheet

Penta-Ubiquitin/Ub5 WT Chains (K48-linked)

Price: $275.00
Catalog #: UC-216B

Penta-Ubiquitin/Ub5 WT Chains (K48-linked)

25 µg
Data Sheet: 

Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules.  Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements.

K48-linked Penta-Ubiquitin chains are manufactured using recombinant wild-type human Ubiquitin and linkage-specific enzymes.  The use of purely enzymatic techniques avoids the potential for contaminating synthetic intermediates.  The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.

Product Information

1 mg/ml (23 µM) in sterile, deionized water
43 kDa
> 90% by SDS-PAGE gel under reducing conditions and visualized by Colloidal Coomassie Blue Stain

Store at -20°C.  Avoid multiple freeze/thaw cycles.