UC-1148

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Material Data Sheet

Tri-Ub/Ub3 WT Chains (K11/K48 “branched”)

Price: $125.00
Catalog #: UC-1148

Tri-Ub/Ub3 WT Chains (K11/K48 “branched”)

Price: 
$125
Quantity: 
25 µg
Data Sheet: 

Linkage specific polyubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Polyubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements. 

K11/K48-branched polyubiquitin chains facilitate rapid substrate delivery to the proteasome, which prevents accumulation of aggregation-prone nascent proteins.  Furthermore, enzymes and effectors of K11/K48-linked chains are encoded by essential genes and mutated across neurodegenerative diseases, providing proof that heterotypic ubiquitin chains play important roles in cellular signaling pathways.

The correctness of linkage and purity of each production lot of this product is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of polyubiquitinn samples.  The product is also tested in a Western Blot application described by Yau, et al.

Product Information

Stock: 
0.5 mg/ml (20 µM) in 10 mM HEPES pH 7.5
MW: 
26 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Ubiquitin chains vary in length, linkage, and function.  K11/K48­linked, “branched” Tri-Ubiquitin chains are ideal as a Western Blot control as described by Yau et al. in “Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control”

Storage: 

Store at –20°C.  Avoid multiple freeze/thaw cycles