UC-101

Material Data Sheet

Di-ubiquitin (Ub2), (K33-linked) 

Price: $250.00
Catalog #: UC-101

Di-ubiquitin (Ub2), (K33-linked) 

Price: 
250
Quantity: 
25 µg
Data Sheet: 
application/pdf iconUC-101_k33 diUb synth_25ug.pdf

 Linkage specific di-Ub is a substrate for enzymes that cleave the isopeptide linkage between two ubiquitin molecules.  It can also be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs).

Product Information

MW: 
17 kDa
Purity: 
> 90% by SDS-PAGE, containing <10% mono-Ubiquitin
Solubility: 
Aqueous solutions up to 10 mg/ml&nbsp;

Use & Storage

Use: 

 Typical concentrations will depend on specific assay conditions and method of detection. 

Storage: 
Solubilized solution at –80°C. &nbsp;Avoid multiple freeze/thaw cycles.&nbsp;

Literature

References: 

Buchberger A., et al. (2002) Trends. Cell. Biol. 12:216-221

Cook W.J., et al. (1992) J. Biol. Chem. 267:16467-16471

El Oualid, F., et al. (2010) Angewandte Chemie. 49: 10149-10153

Fischer R.D., et al. (2003) J. Biol. Chem. 278:28976-28984

Tenno T., et al. (2004) Genes to Cells.  9:865-875

Varadan R., et al. (2002) J. Mol. Biol. 324:637-647

Varadan R., et al. (2004) J. Biol. Chem. 279:7055-7063