UBE-235

Material Data Sheet

RAP80 UIM Domains Biotin

Price: $245.00
Catalog #: UBE-235

RAP80 UIM Domains Biotin

Price: 
$245
Quantity: 
250 µg

Rap80 (Receptor Associated Protein 80) interacts with BRCA1, a ubiquitin E3-ligase which functions in conjugation with the BARD1 deubiquitinating enzyme. BRCA1 is recruited to DNA damage sites by poly-ubiquitin chains through Rap80 which contains 2 tandem ubiquitin-interacting motifs (UIMs). Rap80 constitutes a protein complex with ABRA1 which interacts with the BRCT domain of BRCA1. Upon DNA damage the Rap80-ABRA1 complex targets the BRCA1-BARD1 complex to K6- and K63-linked poly-Ub chains at these foci. The UIM domains of Rap80 have been shown to have preferential binding to K-6 and K63-linked Ub chains and binds to K48-chain with a much lower efficiency. It is not known if Rap80 UIMs interact with Ub chains linked via K11, K27 or K33. Rap80 has a low affinity for mono-, di- and tri-Ub but binds efficiently to tetra (or greater) ubiquitin chains. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with other antibodies.

Product Information

Stock: 
X mg/ml (X μM) 50 mM Hepes, 250 mM NaCl, 1 mM DTT pH 8.0
Purity: 
>95% by SDS-PAGE

Use & Storage

Use: 

Use 50-100 μg of protein to detect 10-20 μg of purified K63-linked ubiquitin chains. The amount necessary for use in crude lysates must be empirically determined.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Buchberger A. (2002) Tren. Cell. Biol. 12 :216-221

Hurley J.H., et al. (2006) Biochem. J. 399 :361-372

Kim H., et al. (2007) Science. 316:1202-1205

Kim H., et all. (2008) Mol. Cells. 25:457-61

Sobhian B., et all. (2007) Science. 316:1198-1202

Wang B., (2007) Science. 316:1194-1198

Wu W., et al. (2008) Cell. Div. 31-10

Yan J., et al. (2007) Canc. Res. 15:6647-6656