UBE-215

Material Data Sheet

HR23A/Rad23A Tandem UBA (TUBE1) Biotin

Price: $245.00
Catalog #: UBE-215

HR23A/Rad23A Tandem UBA (TUBE1) Biotin

Price: 
$245
Quantity: 
250 µg
Data Sheet: 

hHR23A has two UBA domains that can each bind Ubiquitin in addition to an N-terminal UBL domain that binds S5a and S2, two components of the 26S proteasome.  hHR23a recognizes Ubiquitin through a predominately hydrophobic surface formed by residues within α1 and α3 of each of its UBA domains. Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-Ubiquitin moieties over the single Ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-Ubiquitinated proteins, allowing for detection at relatively low abundance. This protein can be used for the enrichment, isolation and identification of K63-linked or K48-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages.

Product Information

Stock: 
X mg/ml (X μM) 50 mM HEPES pH 8.0, 200 mM NaCl, 2 mM TCEP
MW: 
28 kDa
Purity: 
>95% by SDS-PAGE

Use & Storage

Use: 

Use 50-100 μg of protein to detect 2-25 μg of purified poly-Ubiquitin chains. The amount necessary for use in crude lysates must be determined empirically.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.