Material Data Sheet

 HR23A/Rad23A Tandem UBA (TUBE1) Fluorescein

Price: $251.00
Catalog #: UBE-212

 HR23A/Rad23A Tandem UBA (TUBE1) Fluorescein

100 µg
Data Sheet: 

hHR23A has two UBA domains that can each bind ubiquitin in addition to an N-terminal UBL domain that binds S5a and S2, two components of the 26S proteasome.  hHR23a recognizes ubiquitin through a predominately hydrophobic surface formed by residues within α1 and α3 of each of its UBA domains. Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of polyubiquitinated proteins. TUBEs display increased affinity for poly-Ubiquitin moieties over the single Ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-Ubiquitinated proteins, allowing for detection at relatively low abundance. This protein contains a single fluorescein dye and an amino-terminal His6 tag.

Product Information

X mg/ml (X mM) 50 mM HEPES pH 7.5, 200 mM NaCl
29 kDa
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Store at -80°C in the dark and avoid multiple freeze/thaw cycles.