UBE-115

Material Data Sheet

Ubiquilin 1 Tandem UBA (TUBE2) Biotin

Price: $245.00
Catalog #: UBE-115

Ubiquilin 1 Tandem UBA (TUBE2) Biotin

Price: 
$245
Quantity: 
250 µg
Data Sheet: 

Ubiquilin-1 contains an N-terminal Ubiquitin-like domain and a C-terminal Ubiquitin-associated domain. It associates with proteasomes and E3 ligases, and is thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. Ubiquilin-1 has been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-Ubiquitin moieties over single Ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-Ubiquitinated proteins, allowing for detection at relatively low abundance. This protein can be used for the isolation and identification of K48-linked (preferentially) or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with other antibodies.

Product Information

Stock: 
X mg/ml (X μM) 50 mM HEPES pH8.0, 200 mM NaCl, 1 mM DTT
MW: 
22 kDa
Purity: 
>95% by SDS-PAGE

Use & Storage

Use: 

Use 50-100 μg of protein to detect 2-25 μg of purified Ubiquitin chains. The amount necessary for use in crude lysates must be determined empirically.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.