UBE-100

Material Data Sheet

His6-Ataxin UIM Domains

Price: $245.00
Catalog #: UBE-100

His6-Ataxin UIM Domains

Price: 
$245
Quantity: 
250 µg

Ataxin-3 protein belongs to a novel group of cysteine proteases similar to USP-type ubiquitin proteases and has deubiquitinating activity in vitro. The full-length protein contains an catalytic N-terminal Josephin domain, three ubiquitin interacting motifs (UIMs), and a variable C-terminus with a polyglutamine stretch. Ataxin3 has deconjugating activity and functions as a mixed linkage, chain editing enzyme with preferential cleavage of K63 linkages in mixed chains. Ataxin3 also bind both K48-linked and K63-linked poly-Ub chains via its UIM domains and preferentially interacts with four or more ubiquitin units. This protein can be used for the isolation and identification of K48-linked (preferentially) or K63-linked poly-Ub chains or ubiquitinated substrates that contain these linkages. This protein is His-tagged which allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His6-specific antibodies.

Product Information

Stock: 
X mg/ml (X μM) 50 mM Hepes, 200 mM NaCl, 1 mM DTT pH 8.0
Purity: 
>95% by SDS-PAGE

Use & Storage

Use: 

Use 50-100 μg of protein to detect 10-20 μg of purified K48-linked ubiquitin chains. The amount necessary for use in crude lysates needs to be determined empirically.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Albrecht., et al. (2004) Eur. J. Biochem. 271:3155-3170

Buchberger A. (2002) Tren. Cell. Biol. 12 :216-221

Burnett B., et al. (2003) Hum. Mol Genet. 12 :3195-3205

Hurley J.H., et al. (2006) Biochem. J. 399 :361-372

Mao Y., et al. (2005) Proc. Natl. Acad. Sci. 102 :12700-12705

Shoesmith S.J., et al. (2005) J. Biol. Chem. 280 :32026-32034