UB-NOK

Material Data Sheet

Ubiquitin No Lys,N-Terminal Biotin

Price: $159.00
Catalog #: UB-NOK

Ubiquitin No Lys,N-Terminal Biotin

Price: 
$159
Quantity: 
50 µg
Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus. This ubiquitin mutant lacks all reactive lysine residues which have been mutated to arginine. These mutations render the protein unable to form poly-ubiquitin chains and can be used as a negative control or to detect mono-ubiquitination. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with antiubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin.

Product Information

Stock: 
Soluble in aqueous buffers up to 5 mg/ml.
MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428