UB-K630

Material Data Sheet

Ubiquitin w/K63 only N-Term Biotin

Price: $215.00
Catalog #: UB-K630

Ubiquitin w/K63 only N-Term Biotin

Price: 
$215
Quantity: 
50 µg
Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin. This ubiquitin mutant contains only a single lysine (K63) with all other lysines mutated to arginine, and is able to form poly-ubiquitin chains with other ubiquitin molecules via the K63 lysine only.

Product Information

Stock: 
Soluble in aqueous buffers up to 20 mg/ml.
MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428