UB-HK480

Material Data Sheet

His6-Ubiquitin w/K48 only N-Term Biotin

Price: $159.00
Catalog #: UB-HK480

His6-Ubiquitin w/K48 only N-Term Biotin

Price: 
$159
Quantity: 
50 µg
Ideal as an alternative to radio-labeled ubiquitin, with detection by avidin-linked reagents. Allows for poly-ubiquitin chain incorporation of biotin-N-terminal ubiquitin with higher efficiency and detection sensitivity than traditionally modified biotin-ubiquitin. Produced via a proprietary process resulting in a single biotin modification exclusively on the N-terminus of ubiquitin. This ubiquitin mutant contains only a single lysine (K48) with all other lysines mutated to arginine, and is able to form poly-ubiquitin chains with other ubiquitin molecules via the K48 lysine only.

Product Information

Stock: 
Soluble in aqueous buffers up to 20 mg/ml.
MW: 
9.3 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Chau V., et al. (1989) Science 243:1576-1583

Corsi D., et al. (1995) J. Biol. Chem. 270 (15):8928-35.

Gregori L., et al. (1990) J. Biol. Chem. 265:8354-8357

Haldeman M.T., et al. (1997) Biochemistry 36:10526

Mitsui A.,et al. (1999) PNAS 96:6054-6059

Pickart C.M., et al. (1997) FASEB J. 11:1055-1066

Varadan R., et al. (2004) J. Biol. Chem. 279:7055-7063