UB-570

New
Material Data Sheet

Ubiquitin Biotin

Price: $159.00
Catalog #: UB-570

Ubiquitin Biotin

Price: 
$159.00
Quantity: 
100 µg
Data Sheet: 
Ubiquitin modified with biotin via primary amine coupling. This results in multiple biotinylated ubiquitin species modified at the N-terminus, as well as lysine residues. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate polyubiquitin chains. Biotinylated ubiquitin can be detected using avidin-linked reagents.

Product Information

Stock: 
X mg/ml in 10 mM HEPES, pH 7.5
MW: 
8.6 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application. We recommend using Biotinylated Recombinant Human Ubiquitin in conjunction with native ubiquitin at a combined concentration of 10-50 μM with a 1:1 to 1:5 ratio of biotinylated ubiquitin:native ubiquitin. The resulting poly-Ubiquitin chains can be visualized or captured with avidin-linked reagents.

Storage: 

Store at -20°C or -80°C