UB-560

Material Data Sheet

Ubiquitin N-Terminal Biotin

Price: $199.00
Catalog #: UB-560

Ubiquitin N-Terminal Biotin

Price: 
$199.00
Quantity: 
50 µg
Data Sheet: 

Produced via a proprietary process resulting in a single biotin modification exclusively on the aminoaterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the carboxy-terminus, and with the full complement of reactive lysines to allow for polyubiquitin chain incorporation.  Detection with avidin-linked reagents allows for efficient detection and/or capture.

Product Information

Stock: 
X mg/ml (X µM) in 10 mM HEPES pH 8.0
MW: 
9 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue Stain
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively.

Storage: 

Store at -20°C or -80°C.  Avoid multiple freeze/ thaw cycles.