U-720

Material Data Sheet

Methylated Ubiquitin- 13C 15N

Price: $195.00
Catalog #: U-720

Methylated Ubiquitin- 13C 15N

Price: 
$195
Quantity: 
100 μg

Isotopically labeled ubiquitin is useful in determining total cellular concentrations of ubiquitin, or determining the ratio of free- to substrate-bound ubiquitin using the protein standard absolute quantification (PSAQ) or related methods.  Reductive methylation of lysine residues renders ubiquitin unable to form poly-ubiquitin chains via lysine linkages with other Ub molecules.  Methylated Ub can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins in a mono-ubiquitinated or multi-mono-ubiquitinated fashion.

Product Information

Stock: 
lyophilized powder
MW: 
9255.5 Da (> 95% labeled)
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 50 mg/ml

Use & Storage

Use: 

Typical amount for use as an internal recovery standard is approximately 1 μg  per gram of protein lysate. 

Storage: 
Lyophilized powder at 4oC. Solubilized stock solution at -20oC. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Ciechanover A., et al. (1980)  J. Biol. Chem. 255: 7525-7528

Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847

Glickman M.H. and Ciechanover A. (2002) Physiol. Rev.  82: 373-428

Hershko A. and Ciechanover A. (1992) Ann. Rev. Biochem. 61: 761-807

Kaieser S., et al.  (2011) Nature Methods 8: 691-696

Schwartz A.L and Ciechanover A. (1999) Ann. Rev. Med. 50: 57-74.

Wilkinson K.D. and Audhya T.K. (1981) J. Biol. Chem. 256: 9235-9241