U-720

Material Data Sheet

Methylated Ubiquitin-13C 15N

Price: $195.00
Catalog #: U-720

Methylated Ubiquitin-13C 15N

Price: 
$195
Quantity: 
100 µg

Isotopically labeled ubiquitin is useful in determining total cellular concentrations of ubiquitin, or
determining the ratio of free- to substrate-bound ubiquitin using the protein standard absolute
quantification (PSAQ) or related methods. Reductive methylation of lysine residues renders ubiquitin
unable to form poly-ubiquitin chains via lysine linkages with other Ub molecules. Methylated Ub can form
an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of
substrate proteins in a mono-ubiquitinated or multi-mono-ubiquitinated fashion.

Product Information

MW: 
9255.5 Da (> 95% labeled)
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 50 mg/ml
Supplied: 
Lyophilized Powder

Use & Storage

Use: 

Typical amount for use as an internal recovery standard is approximately 1 μg per gram of protein lysate

Storage: 
Lyophilized powder at 4 Degrees C. Solubilized stock solution at -20 Degrees C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ciechanover A., et al. (1980) J. Biol. Chem. 255: 7525-7528
Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847
Glickman M.H. and Ciechanover A. (2002) Physiol. Rev. 82:373-428
Hershko A. and Ciechanover A. (1992) Ann. Rev. Biochem. 61: 761-807
Kaieser S., et al. (2011) Nature Methods 8: 691-696
Schwartz A.L and Ciechanover A. (1999) Ann. Rev. Med. 50: 57-74.
Wilkinson K.D. and Audhya T.K. (1981) J. Biol. Chem. 256: 9235-9241