U-700

Material Data Sheet

Ubiquitin-13C 15N

Price: $119.00
Catalog #: U-700

Ubiquitin-13C 15N

Price: 
$119
Quantity: 
100 µg

Isotopically labeled ubiquitin is useful in determining total cellular concentrations of ubiquitin, or determining the ratio of free ubiquitin to poly-ubiquitin chains using the protein standard absolute quantification (PSAQ) or related methods.  Highly purified 13C/15N labeled ubiquitin processed for the quantitative removal of glycine and buffer salts which can interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. 

Product Information

MW: 
9034.6 Da (> 98% labeled)
Purity: 
> 95% by SDS-PAGE
Solubility: 
Aqueous solutions up to 50 mg/ml

Use & Storage

Use: 
Typical amount for use as an internal recovery standard is approximately 1 g per gram of protein lysate
Storage: 
Lyophilized powder at 4oC. Solubilized stock solution at -20oC. Avoid multiple freeze/thaw cycles.

Literature

References: 

Ciechanover A., et al. (1980)  J. Biol. Chem. 255: 7525-7528

Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847

Glickman M.H. and Ciechanover A. (2002) Physiol. Rev.  82:373-428

Hershko A. and Ciechanover A. (1992) Ann. Rev. Biochem. 61: 761-807

Kaieser S., et al.  (2011) Nature Methods 8: 691-696

Schwartz A.L and Ciechanover A. (1999) Ann. Rev. Med. 50: 57-74.

Wilkinson K.D. and Audhya T.K. (1981) J. Biol. Chem. 256: 9235-9241