U-590

Material Data Sheet

Ubiquitin Fluorescein

Price: $139.00
Catalog #: U-590

Ubiquitin Fluorescein

Price: 
$139
Quantity: 
100 µg
Ubiquitin modified with fluorescein via primary amine coupling. This results in multiple fluoresceinated ubiquitin species with modified lysines as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to efficiently incorporate into poly-ubiquitin chains.

Product Information

Stock: 
Soluble in aqueous buffer or DMSO up to 20 mg/ml.
MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Fluorescein-ubiquitin gives a strong signal in the range of 0.1-1 µM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively. 

Storage: 
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256:9235-9241