U-580

Material Data Sheet

Ubiquitin N-Terminal Fluorescein

Price: $159.00
Catalog #: U-580

Ubiquitin N-Terminal Fluorescein

Price: 
$159
Quantity: 
50 µg
Produced via a proprietary process resulting in a single fluorescein modification exclusively on the N-terminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. This reagent allows for poly-ubiquitin chain incorporation of fluorescein-N-terminal ubiquitin with higher efficiency and detection sensitivity than traditionally modified ubiquitins.

Product Information

Stock: 
Reconstitute in aqueous buffer or DMSO at 1 mg/ml
MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 
lyophilized powder

Use & Storage

Use: 

Fluorescein-N-term-ubiquitin gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions.  Optimal fluorescence at pH 8.0 is monitored using excitation and emission wavelengths of 490nm and 515 nm, respectively. 

Storage: 

Store at -20°C once reconstituted.  Avoid multiple freeze/ thaw cycles.

Literature

References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428

Wilkinson K.D. and Audhya T.K. (1981) J.Biol.Chem. 256:9235-9241