U-570

Material Data Sheet

Ubiquitin Biotin

Price: $145.00
Catalog #: U-570

Ubiquitin Biotin

Price: 
$145
Quantity: 
100 µg
Ubiquitin modified with biotin via primary amine coupling. This results in multiple biotinylated ubiquitin species modified at the N-terminus, as well as lysine residues. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate polyubiquitin chains. Biotinylated ubiquitin can be detected using avidin-linked reagents.

Product Information

Stock: 
Soluble in aqueous buffers up to 50 mg/ml.
MW: 
8.6 kDa
Purity: 
> 95% by PAGE
Supplied: 
This is a lyophilized powder

Use & Storage

Use: 

Depending on desired assay signal and conditions, biotin-Ub should be used in conjunction with native ubiquitin with a 1:1 to 1:5 ratio of biotin-Ub: native Ub, respectively, with a total combined concentration range of 100-500 μM. Typical amounts for a 20 μL reaction: 5 μg - 15 μg biotin-Ub, 20 μg native ubiquitin. Assessment of ubiquitination performed via avidin-linked detection.

Storage: 

Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.

Literature

References: 

Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935

Mitsui A., et al. (1999) PNAS 96:6054-6059

Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428