- Home
- About
- Products
- E1 Activating
- E2 Conjugating
- E3 Ligase
- Deubiquitinating Enzymes (DUBs)
- Deconjugating Enzymes
- Proteasome
- Inhibitors
- Ubiquitin
- All
- Ubiquitin and Ubiquitin Derivatives
- Ubiquitin Chains
- DUB-Resistant, Non-Hydrolyzable Chains
- NH Isopeptide-linked chains
- DCA Linked Chains
- K6-linked Chains (DCA)
- Agarose Bound K6-linked Chains (DCA)
- K11-linked Chains (DCA)
- Agarose Bound K11-linked Chains (DCA)
- K29-linked Chains (DCA)
- Agarose Bound K29-linked Chains (DCA)
- K33-linked Chains (DCA)
- Agarose Bound K33-linked Chains (DCA)
- K48-linked Chains (DCA)
- Agarose Bound K48-linked Chains (DCA)
- K63-linked Chains (DCA)
- Agarose Bound K63-linked Chains (DCA)
- K76-linked Chains (DCA)
- Agarose Bound K76-linked Chains (DCA)
- Ubiquitin Mutants
- Labeled Ubiquitin Proteins
- Antibodies, ELISAs and Kits
- SUMO
- NEDD8
- ISG15
- UFM1
- URM1
- Autophagy
- FAT10
- Antibodies
- Kits
- Buffers, Solutions, Standards
- Substrate Proteins
- Affinity Matrices / Proteins
- Fractions
- FUBI
- References
- Orders
- Contact
Material Data Sheet
His6-Ubiquitin N-Terminal Biotin
Price: $150.00
His6-Ubiquitin N-Terminal Biotin
Price:
150
Quantity:
50 µg
Data Sheet:
Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin
chain incorporation. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to
radio-labeled ubiquitin.
Product Information
Stock:
Soluble in aqueous buffers up to 20 mg/ml.
MW:
8.6 kDa
Purity:
> 95% by PAGE
Supplied:
This is a lyophilized powder Use & Storage
Use:
Depending on desired signal strength and assay conditions, biotin-N-terminal Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-N-term-Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.
Storage:
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Literature
References:
Corsi D., et al. (1995) J.Biol.Chem. 270:8928-8935
Mitsui A., et al. (1999) PNAS 96:6054-6059
Mimnaugh E.G. et al. (1999) Electrophoresis 29:418-428






